Regulatory

Part:BBa_K319031:Design

Designed by: Matthew Orton   Group: iGEM10_uOttawa   (2010-10-20)

yeast L20 Proximal Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 162
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was designed using primers that introduced standard 20 bp primer binding sites flanking the upstream and downstream regions of the part.



Source

The part was amplified via colony PCR from a YPH500 strain of S. cerevisiae. The strain expressing the part was given to us by Tom Ellis.


References

Ellis T, Wang X and Collins JJ. Diversity-based, model-guided construction of synthetic gene networks with predicted functions. Nature Biotechnology 27: 465-471 (2009).